*All times are CET
Program Day 1 – February 15th 2023
13:30 Welcome from the IOC 2023
Session 1: Emerging technologies/methodologies for uEV isolation, quantification, and profiling
13:40 Chairs: Fabrice Lucien and Robert Myette
13:45 Plenary Talk: David Greening, PhD, Molecular Proteomics Division, Baker Heart & Diabetes Institute, Australia Extracellular vesicle proteome complexity and diagnostic potential
Featured Abstract
14:10 Xiao-Xiao Zhu, China, Identification of stable reference genes for relative quantification of long RNA expression in urinary exosomes
14:25 Yohan Kim, USA, Automation of clinical urinary extracellular vesicle measurement using standardized and calibrated nanoscale flow cytometry
14:40 Cristina Grange, Italy, Super resolution microscopy for single EV evaluation: better understanding of urinary EV subpopulations
14:55 Beatriz Martín-Gracia, Norway, Ultrasensitive detection of urinary prostate cancer EVs using gold nanoprisms as thermal transductors in lateral flow immunoassays
15:10 Panel Discussion: Are EV technologies ready for the clinic?
Moderator: Fabrice Lucien. Panelists: Leonora Balaj (USA), Edwin van der Pol (Netherlands), Shannon Stott (USA)
15:40 00:30 BREAK
Session 2: Urinary EV collection, handling and storage
16:10 Chairs: Maija Puhka and Martin Van Royen
16:15 Plenary Talk: Elena Martens-Uzunova, Department of Urology, Rotterdam, The Netherlands, Quality collection and biobanking for urinary EVs
Featured Abstract
16:40 Karina Barreiro, Finland, Capturing the kidney transcriptome by urinary EV – impact of cryostorage temperature and EV isolation
16:55 Adele Tanzi, Italy, Urinary Extracellular vesicle preservation using a non-toxic dialdehyde fixative
17:10 Jenni Karttunen, Finland, Identification of the most stable and vulnerable – Impact of preanalytical variables on the uEV miRNome
17:25 Andres Lõhmus, Estonia, Tangential-flow filtration provides an easy and robust method for the isolation of urinary EVs with high yield and purity index
17:40 Introduction of the Keynote: Maija Puhka
17:45 Keynote: Prof. Per-Henrik Groop, MD, DMSc, Professor of Internal Medicine, Department of Nephrology, and Tiinamaija Tuomi, MD, PhD, Associate professor, Institute for Molecular Medicine Finland FIMM, University of Helsinki. Considerations for long-term biomarker studies – focus on diabetic kidney disease
18:30 Panel Discussion: How to improve the quality of uEV research?
Moderator: Maija Puhka. Panelists: Peter Yuen, Elena Martens-Uzunova, Per-Henrik Groop, Tiinamaija Tuomi
19:00 Closing Day 1: IOC 2023
Day 2 – February 16th 2023
13:30 Welcome from the IOC 2023 – Wrap up Day 1
Session 3: Roles of urinary EVs in (patho)physiology
13:40 Update from the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV: looking ahead (Uta Erdbruegger and Metka Lenassi)
13:50 Chairs: Cristina Grange and Per Svenningsen
13:55 Plenary Talk: Laura Perin, Division of Urology, University of Southern California, USA. In vitro and in vivo model to study kidney disease
Featured abstract
14:20 Song-Tao Feng, China, Profiling long RNA transcriptome and tracking cell origin for urinary extracellular vesicles
14:35 Samantha Upson, USA, Daily variability of urinary extracellular vesicles in healthy subjects
14:50 Qian Chen, UK, Characterisation of urine small extracellular vesicles from normal pregnancy and early-onset preeclampsia
15:05 Danilo Bondi, Italy, Biophysical and biomolecular uEVs signatures distinguish inactive versus endurance-trained male adults
15:20 Panel Discussion: Are uEVs waste or functional?
Moderator: Benedetta Bussolati. Panelists: Lin-Li Lv, Ewout Hoorn, Per Svenningsen, Kenneth Witwer
15:50 BREAK
Session 4: Urinary EV as biomarkers in human health and disease
Chairs: Janne Leivo and Uta Erdbruegger
16:25 Plenary Talk: Francesc Enric Borras Serres IGTP and Nephrology Department, University Hospital Germans Trias i Pujol (HUGTiP), Spain. Urine Biomarker discovery for kidney fibrosis: a long & winding road
Featured abstracts
16:50 Amanda Khoo, Canada, Proteomic characterization of uEVs identifies uEV-specific protein signatures for improved risk stratification in localized prostate cancer
17:05 Mei He, USA, Isolation and Characterization of Urinary Extracellular Vesicles from Bladder Cancer Patients
17:20 Irene Bijnsdorp, The Netherlands, Detecting prostate cancer in urinary EVs making use of the surface proteome of (cancer) EVs
17:35 Loic Steiner, Sweden, The immunomodulatory role of Extracellular Vesicles in Urinary Bladder Cancer and their use as a clinical prognostic tool
17:50 Introduction of the Keynote: Fabrice Lucien
17:55 Keynote: Prof. Johan Skog, USA, Chief Scientific Officer and Vice-President at ExosomeDx. Exosome biomarkers: from bench to bedside
18:40 Panel Discussion: Which EV cargo is the better biomarker?
Moderator: Janne Leivo. Panelists: Johan Skog, Dylan Burger, Irene Binjnsdorp, Juan Falcón-Pérez
19:10 Closing Day 2: IOC 2023 and Award ceremony