*All times are CET

Program Day 1 – February 15th 2023

13:30   Welcome from the IOC 2023

Session 1: Emerging technologies/methodologies for uEV isolation, quantification, and profiling

13:40    Chairs:  Fabrice Lucien and Robert Myette

13:45   Plenary Talk:  David Greening, PhD, Molecular Proteomics Division, Baker Heart & Diabetes Institute, Australia Extracellular vesicle proteome complexity and diagnostic potential

Featured Abstract      

14:10    Xiao-Xiao Zhu, China, Identification of stable reference genes for relative quantification of long RNA expression in urinary exosomes

14:25    Yohan Kim, USA, Automation of clinical urinary extracellular vesicle measurement using standardized and calibrated nanoscale flow cytometry

14:40    Cristina Grange, Italy, Super resolution microscopy for single EV evaluation: better understanding of urinary EV subpopulations

14:55    Beatriz Martín-Gracia, Norway, Ultrasensitive detection of urinary prostate cancer EVs using gold nanoprisms as thermal transductors in lateral flow immunoassays     

15:10    Panel Discussion:  Are EV technologies ready for the clinic?

Moderator: Fabrice Lucien. Panelists: Leonora Balaj (USA), Edwin van der Pol (Netherlands), Shannon Stott (USA)

15:40    00:30    BREAK

Session 2: Urinary EV collection, handling and storage

16:10    Chairs: Maija Puhka and Martin Van Royen

16:15   Plenary Talk: Elena Martens-Uzunova, Department of Urology, Rotterdam, The Netherlands, Quality collection and biobanking for urinary EVs

Featured Abstract

16:40    Karina Barreiro, Finland, Capturing the kidney transcriptome by urinary EV – impact of cryostorage temperature and EV isolation

16:55    Adele Tanzi, Italy, Urinary Extracellular vesicle preservation using a non-toxic dialdehyde fixative

17:10    Jenni Karttunen, Finland, Identification of the most stable and vulnerable – Impact of preanalytical variables on the uEV miRNome

17:25    Andres Lõhmus, Estonia, Tangential-flow filtration provides an easy and robust method for the isolation of urinary EVs with high yield and purity index

17:40   Introduction of the Keynote: Maija Puhka

17:45    Keynote:  Prof. Per-Henrik Groop, MD, DMSc, Professor of Internal Medicine, Department of Nephrology, and Tiinamaija Tuomi, MD, PhD, Associate professor, Institute for Molecular Medicine Finland FIMM, University of Helsinki. Considerations for long-term biomarker studies – focus on diabetic kidney disease 

18:30   Panel Discussion: How to improve the quality of uEV research?

Moderator: Maija Puhka. Panelists: Peter Yuen, Elena Martens-Uzunova, Per-Henrik Groop, Tiinamaija Tuomi

19:00    Closing Day 1: IOC 2023

Day 2 – February 16th 2023

13:30    Welcome from the IOC 2023 – Wrap up Day 1

Session 3: Roles of urinary EVs in (patho)physiology

13:40    Update from the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV:  looking ahead (Uta Erdbruegger and Metka Lenassi)

13:50    Chairs: Cristina Grange and Per Svenningsen

13:55    Plenary Talk: Laura Perin, Division of Urology, University of Southern California, USA. In vitro and in vivo model to study kidney disease

Featured abstract

14:20    Song-Tao Feng, China, Profiling long RNA transcriptome and tracking cell origin for urinary extracellular vesicles

14:35    Samantha Upson, USA, Daily variability of urinary extracellular vesicles in healthy subjects

14:50    Qian Chen, UK, Characterisation of urine small extracellular vesicles from normal pregnancy and early-onset preeclampsia

15:05    Danilo Bondi, Italy, Biophysical and biomolecular uEVs signatures distinguish inactive versus endurance-trained male adults       

15:20    Panel Discussion: Are uEVs waste or functional?

Moderator: Benedetta Bussolati. Panelists: Lin-Li Lv, Ewout Hoorn, Per Svenningsen, Kenneth Witwer

15:50    BREAK

Session 4: Urinary EV as biomarkers in human health and disease

Chairs: Janne Leivo and Uta Erdbruegger

16:25    Plenary Talk: Francesc Enric Borras Serres  IGTP  and Nephrology Department, University Hospital Germans Trias i Pujol (HUGTiP), Spain. Urine Biomarker discovery for kidney fibrosis: a long & winding road

Featured abstracts

16:50    Amanda Khoo, Canada, Proteomic characterization of uEVs identifies uEV-specific protein signatures for improved risk stratification in localized prostate cancer

17:05    Mei He, USA, Isolation and Characterization of Urinary Extracellular Vesicles from Bladder Cancer Patients

17:20    Irene Bijnsdorp, The Netherlands, Detecting prostate cancer in urinary EVs making use of the surface proteome of (cancer) EVs

17:35    Loic Steiner, Sweden, The immunomodulatory role of Extracellular Vesicles in Urinary Bladder Cancer and their use as a clinical prognostic tool

17:50    Introduction of the Keynote: Fabrice Lucien

17:55    Keynote:  Prof. Johan Skog, USA, Chief Scientific Officer and Vice-President at ExosomeDx. Exosome biomarkers: from bench to bedside

18:40   Panel Discussion: Which EV cargo is the better biomarker?

Moderator: Janne Leivo. Panelists: Johan Skog, Dylan Burger, Irene Binjnsdorp, Juan Falcón-Pérez

19:10    Closing Day 2: IOC 2023 and Award ceremony